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Amersham Life Sciences Inc pvdf membranes
Pvdf Membranes, supplied by Amersham Life Sciences Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pvdf+membrane/hybond+membrane+n/pmc13276156-268-31-33
Average 86 stars, based on 1 article reviews
pvdf membranes - by Bioz Stars, 2026-10
86/100 stars

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Membrane:

Article Title: TGM2-mediated serotonylation of GPX4 confers ferroptosis resistance to promote gastric tumorigenesis.
Article Snippet: .. Next, the samples were separated on SDS‒PAGE gels, transferred to a PVDF membrane (Amersham), and further incubated with the indicated primary antibodies. .. The primary antibodies and dilutions used for western blotting were as follows: anti-Flag (Sigma‒Aldrich, F3165, 1:4000), anti-GPX4 (Abcam, ab125066, 1:5000), anti-TGM2 (ABclonal, A21184, 1:2000), anti-Myc (Santa Cruz, 9E10): sc-40, 1:1000), anti-TFRC (CST, #13113), GAPDH (Yeasen, 30201ES20, 1:2000), anti-β-actin (Sigma‒Aldrich, A2228, 1:4000), and antiHRP-streptavidin (Yeasen, 35105ES60, 1:8000).

Article Title: Procollagen 1 assembles into phase-separated condensates in the endoplasmic reticulum.
Article Snippet: .. Proteins were transferred onto a 0.45-μm PVDF membrane (10600023; Amersham) for 3 h at a constant voltage of 80 V and blocked in 3% BSA in TBST. .. The membrane was incubated with primary antibodies overnight and washed 3 times for 5 min each with TBST before being incubated with secondary antibodies for 1 h. An enhanced chemiluminescence signal was generated by incubating the membranes with Immobilon Forte Western HRP Substrate (WBLUF0100; Millipore) and imaged on an iBright imaging system (Thermo Fisher Scientific).

Article Title: Application of D4 Fluorescent Probes for Quantitative and Spatial Analysis of Cholesterol in Cells
Article Snippet: .. Prior to loading onto a NuPAGETM 4–12% Bis-Tris gel (Invitrogen, Waltham, MA, USA), samples were boiled for 10 min at 70 °C for IP, or, 5 min 90 °C for other purpose, and subsequently transferred to PVDF membrane (co-IP experiment; Amersham TM Hybond; GE Healthcare, Chicago, IL, USA) activated with 100% methanol (HoneywellTM Riedel de-Haen), or nitrocellulose membrane (GE Healthcare, Chicago, IL, USA). .. Membranes were blocked in Tris-buffered saline containing 0.1% Tween-20 (TBS-T) and 5% Bovine Serum Albumin (BSA) (Biowest, Nuaillé, France) for at least 30 min at room temperature.

Article Title: Copper Overload Affects α-Synuclein Clearance Mechanisms in a Parkinson's Disease In Vitro Model.
Article Snippet: .. After the electrophoretic separation, proteins ere transferred onto a PVDF membrane (Amersham Protean, E Healthcare, Chalfont Buckinghamshire, UK). .. The membrane as blocked in Blotto A (Santa Cruz Biotechnology, Dallas, TX, SA) for 1 h, rinsed, and incubated with a primary antibody vernight at 4◦C.

Article Title: Calmodulin requires calcium to be a constitutive component of the spindle pole bodies in fission yeast
Article Snippet: After being centrifuged at 15K rpm for 1min, 200μl of supernatant was collected and 20μl was used for SDS-PAGE gel electrophoresis (Mini-PROTEIN TGX 10% precast, BioRad #4561033, Irvine, CA, USA). .. The gels were either stained with Brilliant blue R-250 (FisherBiotech cat# 6104-59-2) or transferred to PVDF membrane (10600023, Amersham) for immunoblots. .. The blots were first incubated with the anti-GFP antibodies (1:1000; #11814460001; Sigma-Roche) overnight at 4°C, followed by horseradish peroxidase–conjugated secondary antibodies (1:10,000; #sc-516102; Santa Cruz) for 1h at room temperature.

Article Title: A specific amount of RamA must be reached to trigger increased expression of AcrAB, enhance efflux, and confer multidrug resistance
Article Snippet: A Bradford assay was done to quantify protein concentration, and 10 μg of protein was electrophoresed on 4%–12 % NuPAGE® Bis-Tris mini gels in NuPAGE® MES SDS running buffer (Life Technologies, UK). .. Proteins were transferred to a PVDF membrane (Amersham) by electrophoresis for 3 h at 4°C, and the membrane was blocked with 5% non-fat milk solution. .. After overnight incubation with polyclonal antibodies for AcrB (a gift from Helen Zgurskaya) at 1:1000 dilution, RamA6×His [InVitrogen, monoclonal Anti-His (MA-21315) at 1:10 000 dilution] or RNA polymerase β-subunit (as a control) [InVitrogen, monoclonal RNA polymerase beta antibody (MA-25425) at 1:1000 dilution], membranes were washed overnight and incubated with an HRP-linked anti-rabbit secondary antibody (GE Healthcare).

Article Title: Hsa-miR-514a-3p suppresses oral squamous cell carcinoma(OSCC) progression by targeting COL1A1.
Article Snippet: Objective(s): The research aimed to investigate the impact of hsa-miR-514a-3p on the proliferation and invasion of oral squamous cell carcinoma (OSCC), and to identify its potential target gene COL1A1.. Design: The expression patterns, functional enrichment (Gene Set Enrichment Analysis, GSEA), and copy number variations (CNV) of miR-514a-3p and COL1A1 were analyzed using public datasets (E_MTAB_8588, GSE75538, TCGA-HNSC, and Human Protein Atlas, HPA).. The expressions of miR-514a-3p and COL1A1 in clinical samples were detected by immunohistochemistry (IHC) and RT-qPCR.

Article Title: Ferroptosis-related mechanisms in prion diseases provide insights into neurodegeneration and reveal therapeutic implications.
Article Snippet: .. Proteins were separated on a 12% polyacrylamide gel and transferred to a PVDF membrane (Amersham, GE10600023) at 100 V for 90 min. .. The membrane was blocked with 5% skim milk (BD Difco, K02383233) for 1.5 h at RT.

Incubation:

Article Title: TGM2-mediated serotonylation of GPX4 confers ferroptosis resistance to promote gastric tumorigenesis.
Article Snippet: .. Next, the samples were separated on SDS‒PAGE gels, transferred to a PVDF membrane (Amersham), and further incubated with the indicated primary antibodies. .. The primary antibodies and dilutions used for western blotting were as follows: anti-Flag (Sigma‒Aldrich, F3165, 1:4000), anti-GPX4 (Abcam, ab125066, 1:5000), anti-TGM2 (ABclonal, A21184, 1:2000), anti-Myc (Santa Cruz, 9E10): sc-40, 1:1000), anti-TFRC (CST, #13113), GAPDH (Yeasen, 30201ES20, 1:2000), anti-β-actin (Sigma‒Aldrich, A2228, 1:4000), and antiHRP-streptavidin (Yeasen, 35105ES60, 1:8000).

Article Title: Hsa-miR-514a-3p suppresses oral squamous cell carcinoma(OSCC) progression by targeting COL1A1.
Article Snippet: Objective(s): The research aimed to investigate the impact of hsa-miR-514a-3p on the proliferation and invasion of oral squamous cell carcinoma (OSCC), and to identify its potential target gene COL1A1.. Design: The expression patterns, functional enrichment (Gene Set Enrichment Analysis, GSEA), and copy number variations (CNV) of miR-514a-3p and COL1A1 were analyzed using public datasets (E_MTAB_8588, GSE75538, TCGA-HNSC, and Human Protein Atlas, HPA).. The expressions of miR-514a-3p and COL1A1 in clinical samples were detected by immunohistochemistry (IHC) and RT-qPCR.

Staining:

Article Title: Calmodulin requires calcium to be a constitutive component of the spindle pole bodies in fission yeast
Article Snippet: After being centrifuged at 15K rpm for 1min, 200μl of supernatant was collected and 20μl was used for SDS-PAGE gel electrophoresis (Mini-PROTEIN TGX 10% precast, BioRad #4561033, Irvine, CA, USA). .. The gels were either stained with Brilliant blue R-250 (FisherBiotech cat# 6104-59-2) or transferred to PVDF membrane (10600023, Amersham) for immunoblots. .. The blots were first incubated with the anti-GFP antibodies (1:1000; #11814460001; Sigma-Roche) overnight at 4°C, followed by horseradish peroxidase–conjugated secondary antibodies (1:10,000; #sc-516102; Santa Cruz) for 1h at room temperature.

Western Blot:

Article Title: Calmodulin requires calcium to be a constitutive component of the spindle pole bodies in fission yeast
Article Snippet: After being centrifuged at 15K rpm for 1min, 200μl of supernatant was collected and 20μl was used for SDS-PAGE gel electrophoresis (Mini-PROTEIN TGX 10% precast, BioRad #4561033, Irvine, CA, USA). .. The gels were either stained with Brilliant blue R-250 (FisherBiotech cat# 6104-59-2) or transferred to PVDF membrane (10600023, Amersham) for immunoblots. .. The blots were first incubated with the anti-GFP antibodies (1:1000; #11814460001; Sigma-Roche) overnight at 4°C, followed by horseradish peroxidase–conjugated secondary antibodies (1:10,000; #sc-516102; Santa Cruz) for 1h at room temperature.

Electrophoresis:

Article Title: A specific amount of RamA must be reached to trigger increased expression of AcrAB, enhance efflux, and confer multidrug resistance
Article Snippet: A Bradford assay was done to quantify protein concentration, and 10 μg of protein was electrophoresed on 4%–12 % NuPAGE® Bis-Tris mini gels in NuPAGE® MES SDS running buffer (Life Technologies, UK). .. Proteins were transferred to a PVDF membrane (Amersham) by electrophoresis for 3 h at 4°C, and the membrane was blocked with 5% non-fat milk solution. .. After overnight incubation with polyclonal antibodies for AcrB (a gift from Helen Zgurskaya) at 1:1000 dilution, RamA6×His [InVitrogen, monoclonal Anti-His (MA-21315) at 1:10 000 dilution] or RNA polymerase β-subunit (as a control) [InVitrogen, monoclonal RNA polymerase beta antibody (MA-25425) at 1:1000 dilution], membranes were washed overnight and incubated with an HRP-linked anti-rabbit secondary antibody (GE Healthcare).



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